Donkey IgG anti-Chicken IgG (H+L)-Biotin, MinX Bo,Go,Gp,Hm,Ho,Hu,Ms,Rb,Rt,Sh, Polyclonal

Product Overview

• Article Name: Donkey IgG anti-Chicken IgG (H+L)-Biotin, MinX Bo,Go,Gp,Hm,Ho,Hu,Ms,Rb,Rt,Sh, Polyclonal
• Biozol Catalog Number: ROC-603-706-C37
• Supplier Catalog Number: 603-706-C37
• Alternative Catalog Number: ROC-603-706-C37
• Manufacturer: Rockland Immunochemicals
• Host: Donkey
• Category: Antikörper
• Application: ELISA,IHC,WB
• Species Reactivity: Gallus
• Immunogen: Chicken IgG whole molecule
• Conjugation: Biotin
• Alternative Names: Donkey anti-Chicken IgG Antibody Biotin Conjugated, Donkey anti-Chicken IgY Antibody Biotin
• Format: IgG
• Target Specificity: IgG (H+L)
• Cross-Adsorption (MinX): Bovine,Goat,Guinea pig,Hamster (all),Equine,Human,Mouse,Rabbit,Rat,Sheep

Product Properties

• Clonality: Polyclonal
• Concentration: 1.0 mg/mL by UV absorbance at 280 nm
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized
• Target: Chicken
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:10,000 - 1:50,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
• Application Notes: Anti-Chicken IgG Biotin Antibody has been tested by ELISA and is suitable for ELISA, Western Blot and Immunohistochemistry applications. Specific conditions for reactivity should be optimized by end user.

Images

Western Blot of Unconjugated Anti-Chicken IgG (H&L) (DONKEY) Antibody (Min X Bv Gt GP Ham Hs Hu Ms Rb Rt & Sh Serum Proteins) (p/n 603-701-C37). Lane M: 3 µl Molecular Ladder. Lane 1: Chicken IgG whole molecule (p/n 003-0102). Lane 2: Chicken IgG F(c) Fragment (p/n 003-0103). Lane 3: Chicken IgG Fab Fragment (p/n 003-0105). Lane 4: Chicken IgM Whole Molecule (p/n 003-0107). Lane 5: Chicken Serum (p/n D302-05). All samples were reduced. Load: 50 ng per lane. Block: MB-070 for 30 min at RT. Primary Antibody: Anti-Chicken IgG (H&L) (DONKEY) Antibody (Min X Bv Gt GP Ham Hs Hu Ms Rb Rt & Sh Serum Proteins) (p/n 603-701-C37) 1:3,000 for 60 min at RT. Secondary antibody: Anti-Donkey IgG (GOAT) Peroxidase Conjugated Antibody (p/n 616-1302) 1:40,000 in MB-070 for 30 min at RT. Predicted/Observed Size: 25 and 72 kDa for Chicken IgY and Serum, 25 kDa for F(c) and Fab, 75 kDa for IgM. Chicken F(c) migrates slightly higher.