Chicken IgG (H&L) Antibody Texas Red(TM) Conjugated Pre-Adsorbed, Donkey, Polyclonal

Product Overview

• Article Name: Chicken IgG (H&L) Antibody Texas Red(TM) Conjugated Pre-Adsorbed, Donkey, Polyclonal
• Biozol Catalog Number: ROC-603-709-C37
• Supplier Catalog Number: 603-709-C37
• Alternative Catalog Number: ROC-603-709-C37
• Manufacturer: Rockland Immunochemicals
• Host: Donkey
• Category: Antikörper
• Application: DOT, WB
• Species Reactivity: Gallus
• Immunogen: Chicken IgG whole molecule
• Conjugation: Texas Red
• Alternative Names: Donkey anti-Chicken IgG Antibody Texas Red(TM) Conjugation, Donkey anti-Chicken IgY Texas Red(TM) Conjugated Antibody

Product Properties

• Clonality: Polyclonal
• Concentration: 1.0 mg/mL by UV absorbance at 280 nm
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized
• Target: Chicken
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: FLISA: 1:10,000 - 1:50,000, IF Microscopy: 1:1,000 - 1:5,000
• Application Notes: Anti-Chicken IgG Texas Red Antibody has been tested by dot blot and western blot. This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for mult

Images

Western Blot of Unconjugated Anti-Chicken IgG (H&L) (DONKEY) Antibody (Min X Bv Gt GP Ham Hs Hu Ms Rb Rt & Sh Serum Proteins) (p/n 603-701-C37). Lane M: 3 µl Molecular Ladder. Lane 1: Chicken IgG whole molecule (p/n 003-0102). Lane 2: Chicken IgG F(c) Fragment (p/n 003-0103). Lane 3: Chicken IgG Fab Fragment (p/n 003-0105). Lane 4: Chicken IgM Whole Molecule (p/n 003-0107). Lane 5: Chicken Serum (p/n D302-05). All samples were reduced. Load: 50 ng per lane. Block: MB-070 for 30 min at RT. Primary Antibody: Anti-Chicken IgG (H&L) (DONKEY) Antibody (Min X Bv Gt GP Ham Hs Hu Ms Rb Rt & Sh Serum Proteins) (p/n 603-701-C37) 1:3,000 for 60 min at RT. Secondary antibody: Anti-Donkey IgG (GOAT) Peroxidase Conjugated Antibody (p/n 616-1302) 1:40,000 in MB-070 for 30 min at RT. Predicted/Observed Size: 25 and 72 kDa for Chicken IgY and Serum, 25 kDa for F(c) and Fab, 75 kDa for IgM. Chicken F(c) migrates slightly higher.