Rabbit IgG anti-Dog IgG (Fc)-unconj., MinX none

Product Overview

• Article Name: Rabbit IgG anti-Dog IgG (Fc)-unconj., MinX none
• Biozol Catalog Number: ROC-604-4103
• Supplier Catalog Number: 604-4103
• Alternative Catalog Number: ROC-604-4103
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Application: ELISA,IHC,WB
• Species Reactivity: Canine
• Immunogen: Dog IgG F(c) fragment
• Conjugation: Unconjugated
• Alternative Names: rabbit anti Dog IgG F(c) Antibody, rabbit anti Dog IgG Fc Antibody
• Format: IgG
• Target Specificity: IgG (Fc)
• Cross-Adsorption (MinX): no cross-adsorbtion

Product Properties

• Clonality: Polyclonal
• Concentration: 2.1 mg/mL by UV absorbance at 280 nm
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Liquid (sterile filtered)
• Target: Dog
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:20,000 - 1:100,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
• Application Notes: Anti-Dog IgG F(c) antibody has been tested by ELISA and is suitable for western blot and immunohistochemistry, as well as other assays requiring lot-to-lot consistency.

Images

ELISA results of purified Rb Anti-Dog IgG F(c) Antibody tested against purified Dog IgG F(c). Each well was coated in duplicate with 1.0 µg of Dog IgG F(c) (red line), Dog IgG (green line) .The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Blocking buffer MB-060-1000, Gt Anti-Rabbit HRP 611-103-122, and TMB-1000 substrate.