Rabbit IgG anti-Goat IgG (H+L)-unconj., MinX none

Catalog Number: ROC-605-4102
Article Name: Rabbit IgG anti-Goat IgG (H+L)-unconj., MinX none
Biozol Catalog Number: ROC-605-4102
Supplier Catalog Number: 605-4102
Alternative Catalog Number: ROC-605-4102
Manufacturer: Rockland Immunochemicals
Host: Rabbit
Category: Antikörper
Application: ELISA,IHC,WB
Species Reactivity: Goat
Immunogen: Goat IgG whole molecule
Conjugation: Unconjugated
Alternative Names: rabbit anti-GOAT IgG Antibody, rabbit anti goat IgG
Format: IgG
Target Specificity: IgG (H+L)
Cross-Adsorption (MinX): no cross-adsorbtion
Clonality: Polyclonal
Concentration: 2.1 mg/mL by UV absorbance at 280 nm
Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form: Liquid (sterile filtered)
Target: Goat
Antibody Type: Secondary Antibody
Application Dilute: ELISA: 1:50,000 - 1:100,000, IHC: 1:1,000 - 1:5,000, WB: 1:10,000 - 1:60,000
Application Notes: Anti-Goat IgG antibody has been tested by ELISA and western blot, and is suitable for immunohistochemistry, as well as other assays requiring lot-to-lot consistency.
Western Blot of Rabbit anti-Goat IgG Antibody. Lane 1: Goat IgG (p/n 005-0102). Load: 50 ng per lane. Primary antibody: Goat antibody at 1:1,000 overnight at 4C. Secondary antibody: DyLight(TM) 649 rabbit secondary antibody at 1:20,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 28 & 55 kDa, 28 & 55 kDa for Goat IgG. Other band(s): None