Rabbit IgG anti-Goat IgG (H+L)-HRPO, MinX Hu, Polyclonal

Catalog Number: ROC-605-4313
Article Name: Rabbit IgG anti-Goat IgG (H+L)-HRPO, MinX Hu, Polyclonal
Biozol Catalog Number: ROC-605-4313
Supplier Catalog Number: 605-4313
Alternative Catalog Number: ROC-605-4313
Manufacturer: Rockland Immunochemicals
Host: Rabbit
Category: Antikörper
Application: ELISA,IHC,WB
Species Reactivity: Goat
Immunogen: Goat IgG whole molecule
Conjugation: HRPO
Alternative Names: Goat IgG (H&L) Antibody, Rb-a-Goat IgG (H&L) Peroxidase conjugated, Goat IgG (H&L) Peroxidase Antibody in rabbit, rabbit anti-goat IgG peroxidase conjugated Secondary Antibody
Format: IgG
Target Specificity: IgG (H+L)
Cross-Adsorption (MinX): Human
Clonality: Polyclonal
Concentration: 1.0 mg/mL by UV absorbance at 280 nm
Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form: Lyophilized
Target: Goat
Antibody Type: Secondary Antibody
Application Dilute: ELISA: 1:20,000 - 1:120,000, IHC: 1:500 - 1:2,500, WB: 1:1,000 - 1:5,000
Application Notes: Anti-Goat IgG (H&L) peroxidase conjugated has been tested by ELISA and western blot and is suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based
Western Blot of Peroxidase conjugated Rabbit anti-Goat IgG antibody. Lane 1: Goat IgG. Lane 2: Human IgG. Load: 50 ng per lane. Primary antibody: none. Secondary antibody: Peroxidase rabbit secondary antibody at 1:5,000 for 60 min at RT. Block: 5% BSA-TBS overnight at 4C. Predicted/Observed size: 55 kDa, 28 kDa for Goat IgG. Other band(s): none.