Chicken IgG anti-Goat IgG (H+L)-Alk. Phos., MinX none, ALP, Gallus, Polyclonal , AP

Product Overview

• Article Name: Chicken IgG anti-Goat IgG (H+L)-Alk. Phos., MinX none, ALP, Gallus, Polyclonal , AP
• Biozol Catalog Number: ROC-605-9502
• Supplier Catalog Number: 605-9502
• Alternative Catalog Number: ROC-605-9502
• Manufacturer: Rockland Immunochemicals
• Host: Gallus
• Category: Antikörper
• Application: ELISA,IHC,WB
• Species Reactivity: Goat
• Immunogen: Goat IgG whole molecule
• Conjugation: Alk. Phos.
• Alternative Names: Chicken anti-Goat IgG Antibody Alkaline Phosphatase Conjugation, Chicken anti-Goat IgG Alk Phos Conjugated Antibody
• Format: IgG
• Target Specificity: IgG (H+L)
• Cross-Adsorption (MinX): no cross-adsorbtion

Product Properties

• Clonality: Polyclonal
• Concentration: 1.0 mg/mL by UV absorbance at 280 nm
• Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol, pH 8.0
• Form: Liquid (sterile filtered)
• Target: Goat
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:2,000 - 1:10,000, IHC: 1:200 - 1:1,000, WB: 1:500 - 1:2,500
• Application Notes: Anti-Goat IgG Alkaline Phosphatase Antibody has been tested by dot blot and ELISA. This product has been assayed against 1.0 ug of Goat IgG in a standard capture ELISA using pNPP (p-nitrophenyl phosphate) code NPP-10 as a substrate for 30 minutes at ro

Images

ELISA Results of Chicken Anti-Goat IgG Antibody Alkaline Phosphatase Conjugated tested against purified Goat IgG. Each well was coated in duplicate with 1.0 µg of Goat IgG (p/n 005-0102). The working dilution of Goat IgG Alk Phos is 1:3700. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP stabilizer (p/n MB-067) and NPP Buffer (p/n NPP-500).