Goat IgG anti-Guinea Pig IgG (Fc)-HRPO, MinX none

Product Overview

• Article Name: Goat IgG anti-Guinea Pig IgG (Fc)-HRPO, MinX none
• Biozol Catalog Number: ROC-606-1303
• Supplier Catalog Number: 606-1303
• Alternative Catalog Number: ROC-606-1303
• Manufacturer: Rockland Immunochemicals
• Host: Goat
• Category: Antikörper
• Application: ELISA,IHC,WB
• Species Reactivity: Guinea pig
• Immunogen: Guinea Pig IgG F(c) fragment
• Conjugation: HRPO
• Alternative Names: goat Anti-Guinea Pig IgG F(c) Antibody Peroxidase Conjugated, goat Anti-Guinea Pig IgG Fc Fragment Antibody HRP Conjugated
• Format: IgG
• Target Specificity: IgG (Fc)
• Cross-Adsorption (MinX): no cross-adsorbtion

Product Properties

• Clonality: Polyclonal
• Concentration: 2.0 mg/mL by UV absorbance at 280 nm
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized
• Target: Guinea Pig
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:10,000 - 1:50,000, IHC: 1:500 - 1:2,500, WB: 1:1,000 - 1:10,000
• Application Notes: Anti-Guinea Pig IgG F(c) Peroxidase Conjugated Antibody has been tested by ELISA. This product has been assayed against 1.0 ug of Guinea Pig IgG in a standard capture ELISA using ABTS (2,2-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code ABTS-10

Images

ELISA Results of Purified Goat Anti-Guinea Pig IgG F(c) Antibody Peroxidase Conjugated tested against purified Guinea Pig IgG F(c). Each well was coated in duplicate with 10 µg of Guinea Pig IgG F(c) (p/n 006-0103). The working dilution of Guinea Pig IgG F(c) HRP is 1:31,000. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% Fish Gel/PBS Blocking buffer (p/n MB-066), Guinea Pig IgG F(c) HRP conjugated and TMB substrate (p/n TMBE-1000).