Goat IgG anti-Guinea Pig IgG (H+L)-Biotin, MinX none

Catalog Number: ROC-606-1602
Article Name: Goat IgG anti-Guinea Pig IgG (H+L)-Biotin, MinX none
Biozol Catalog Number: ROC-606-1602
Supplier Catalog Number: 606-1602
Alternative Catalog Number: ROC-606-1602
Manufacturer: Rockland Immunochemicals
Host: Goat
Category: Antikörper
Application: ELISA,IHC,WB
Species Reactivity: Guinea pig
Immunogen: Anti-Guinea Pig IgG (H&L) was produced by repeated immunization with guinea pig whole molecule in goat.
Conjugation: Biotin
Alternative Names: Guinea pig IgG (H&L) Antibody, Gt-a-Guinea pig Biotin conjugated, Guinea pig IgG (H&L) Antibody in goat, Goat IgG (H&L) Biotin conjugated Secondary Antibody.
Format: IgG
Target Specificity: IgG (H+L)
Cross-Adsorption (MinX): no cross-adsorbtion
Clonality: Polyclonal
Concentration: 2.0 mg/mL by UV absorbance at 280 nm
Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form: Lyophilized
Target: Guinea Pig
Antibody Type: Secondary Antibody
Application Dilute: ELISA: 1:20,000 - 1:100,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
Application Notes: Anti-Guinea Pig (H&L) biotin conjugated antibody generated in goat detects specifically guinea pig IgG (H&L). This secondary biotin conjugated antibody anti-Guinea Pig has been tested by ELISA and is ideal for investigators who routinely perform titratio
ELISA Results of Goat Anti-Guinea Pig IgG Antibody Biotin Conjugated tested against purified Guinea Pig IgG Biotin. Each well was coated in duplicate with 1.0 µg of Guinea Pig IgG (p/n 006-0102). The working dilution is 1:81,000. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP Conjugation Buffer (p/n MB-076), Streptavidin-HRP conjugated (p/n S000-03), and TMB substrate (p/n TMBE-1000).