Rabbit IgG anti-Guinea Pig IgG (Fc)-Alk. Phos., MinX none, ALP, Polyclonal , AP

Product Overview

• Article Name: Rabbit IgG anti-Guinea Pig IgG (Fc)-Alk. Phos., MinX none, ALP, Polyclonal , AP
• Biozol Catalog Number: ROC-606-4503
• Supplier Catalog Number: 606-4503
• Alternative Catalog Number: ROC-606-4503
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Application: ELISA,IHC,WB
• Species Reactivity: Guinea pig
• Immunogen: Guinea Pig IgG F(c) fragment
• Conjugation: Alk. Phos.
• Alternative Names: Rabbit Anti-Guinea Pig IgG F(c) Antibody Alkaline Phosphatase Conjugated, Rabbit Anti-Guinea Pig IgG Fc Fragment Antibody alk phos Conjugated
• Format: IgG
• Target Specificity: IgG (Fc)
• Cross-Adsorption (MinX): no cross-adsorbtion

Product Properties

• Clonality: Polyclonal
• Concentration: 1.0 mg/mL by UV absorbance at 280 nm
• Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol, pH 8.0
• Form: Liquid (sterile filtered)
• Target: Guinea Pig
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:5,000 - 1:15,000, IHC: 1:200 - 1:1,000, WB: 1:500 - 1:2,500
• Application Notes: Anti-Guinea Pig IgG F(c) Alkaline Phosphatase conjugate has been tested by ELISA and dot blot and is suitable for immunoblotting (western or dot blot), ELISA, immunoelectron microscopy and immunohistochemistry as well as other antibody-based enzymatic as

Images

ELISA Results of Rabbit Anti-Guinea Pig IgG F(c) Antibody Alkaline Phosphatase Conjugated tested against purified Guinea Pig IgG F(c) Alk Phos. Each well was coated in duplicate with 1.0 µg of Guinea Pig IgG F(c) (p/n 006-0103). The working dilution is 1:18,000. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Alkaline Phosphatase ELISA Substrate Buffer (p/n NPP-10) and NPP Working Buffer (p/n NPP-B500).