0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form:
Lyophilized
Target:
Guinea Pig
Antibody Type:
Secondary Antibody
Application Dilute:
ELISA: 1:10,000 - 1:50,000, WB: 1:1,000 - 1:5,000
Application Notes:
Anti-Guinea Pig IgG Peroxidase conjugate has been tested by ELISA and is suitable for immunoblotting (western or dot blot), ELISA, immunoelectron microscopy and immunohistochemistry as well as other antibody-based enzymatic assays requiring lot-to-lot co
ELISA Results of Donkey Anti-Guinea Pig IgG mx10 Antibody Peroxidase Conjugated. Each well was coated in duplicate with 1.0 µg of Guinea Pig IgG (p/n 006-0102). The working dilution is 1:147,000. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using TMBE-1000 substrate.
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