Goat IgG anti-Horse IgG (Fc)-HRPO, MinX none

Product Overview

• Article Name: Goat IgG anti-Horse IgG (Fc)-HRPO, MinX none
• Biozol Catalog Number: ROC-608-1303
• Supplier Catalog Number: 608-1303
• Alternative Catalog Number: ROC-608-1303
• Manufacturer: Rockland Immunochemicals
• Host: Goat
• Category: Antikörper
• Application: ELISA,IHC,WB
• Species Reactivity: Equine
• Immunogen: Horse IgG F(c) fragment
• Conjugation: HRPO
• Alternative Names: goat Anti-Horse IgG F(c) Antibody Peroxidase Conjugated, goat Anti-Horse IgG Fc Fragment Antibody peroxidase Conjugation, goat Anti-Horse IgG Fc HRP Conjugated Antibody
• Format: IgG
• Target Specificity: IgG (Fc)
• Cross-Adsorption (MinX): no cross-adsorbtion

Product Properties

• Clonality: Polyclonal
• Concentration: 2.0 mg/mL by UV absorbance at 280 nm
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized
• Target: Horse
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:10,000 - 1:50,000, IHC: 1:500 - 1:2,500, WB: 1:1,000 - 1:10,000
• Application Notes: Anti-Horse IgG F(c) Peroxidase conjugate has been tested by ELISA and is suitable for immunoblotting (western or dot blot), ELISA, immunoelectron microscopy and immunohistochemistry as well as other antibody-based enzymatic assays requiring lot-to-lot co

Images

ELISA results of purified Goat Anti-Horse IgG F(c) Peroxidase Conjugated Antibody tested against purified Horse IgG F(c). Each well was coated in duplicate with 1.0 µg of Horse IgG F(c) (blue line). The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using TMB-1000 substrate.