Goat IgG anti-Horse IgG (H+L)-Alk. Phos., MinX none, ALP, Polyclonal , AP

Product Overview

• Article Name: Goat IgG anti-Horse IgG (H+L)-Alk. Phos., MinX none, ALP, Polyclonal , AP
• Biozol Catalog Number: ROC-608-1502
• Supplier Catalog Number: 608-1502
• Alternative Catalog Number: ROC-608-1502
• Manufacturer: Rockland Immunochemicals
• Host: Goat
• Category: Antikörper
• Application: ELISA,IHC,WB
• Species Reactivity: Equine
• Immunogen: Horse IgG whole molecule
• Conjugation: Alk. Phos.
• Alternative Names: goat Anti-Horse IgG alkaline phosphatase Conjugated Antibody, goat Anti-Horse IgG Antibody alk phos Conjugation
• Format: IgG
• Target Specificity: IgG (H+L)
• Cross-Adsorption (MinX): no cross-adsorbtion

Product Properties

• Clonality: Polyclonal
• Concentration: 1.0 mg/mL by UV absorbance at 280 nm
• Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol, pH 8.0
• Form: Liquid (sterile filtered)
• Target: Horse
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:2,000 - 1:10,000, IHC: 1:200 - 1:1,000, WB: 1:500 - 1:2,500
• Application Notes: Anti-Horse IgG Alkaline Phosphatase Antibody has been tested by ELISA and dot blot. This product has been assayed against 1.0 ug of Horse IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate code NPP-10 as a substrate for 30 minutes at ro

Images

ELISA Results of Goat Anti-Horse IgG Antibody Alkaline Phosphatase Conjugated tested against purified Horse IgG. Each well was coated in duplicate with 1.0 µg of Horse IgG (p/n 008-0102). The working dilution is 1:6000. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using NPP ELISA Alk Phos Substrate (p/n NPP-100), NPP Buffer (NPP-B500), Horse IgG Alk Phos conjugate, and TMB substrate (p/n TMBE-1000).