Goat IgG anti-Human IgG (H+L)-unconj., MinX Bo,Ck,Go,Gp,Hm,Ho,Ms,Rb,Rt,Sh, Polyclonal , Unconjugated

Product Overview

• Article Name: Goat IgG anti-Human IgG (H+L)-unconj., MinX Bo,Ck,Go,Gp,Hm,Ho,Ms,Rb,Rt,Sh, Polyclonal , Unconjugated
• Biozol Catalog Number: ROC-609-101-123
• Supplier Catalog Number: 609-101-123
• Alternative Catalog Number: ROC-609-101-123
• Manufacturer: Rockland Immunochemicals
• Host: Goat
• Category: Antikörper
• Application: ELISA,IHC,WB
• Species Reactivity: Human
• Immunogen: Human IgG whole molecule
• Conjugation: Unconjugated
• Alternative Names: Goat Anti Human IgG Antibody Pre-Adsorbed, Goat Anti-Human IgG Antibody Pre-Adsorbed
• Format: IgG
• Target Specificity: IgG (H+L)
• Cross-Adsorption (MinX): Bovine,Gallus,Goat,Guinea pig,Hamster (all),Equine,Mouse,Rabbit,Rat,Sheep

Product Properties

• Clonality: Polyclonal
• Concentration: 1.18 mg/mL by UV absorbance at 280 nm
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Liquid (sterile filtered)
• Target: Human
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:20,000 - 1:100,000, IHC: 1:1,000 - 1:2,000, WB: 1:2,000 - 1:10,000
• Application Notes: Anti-Human IgG antibody has been tested by ELISA and is suitable for western blot and immunohistochemistry, as well as other assays requiring lot-to-lot consistency.

Images

ELISA Results of Goat Anti-Human IgG Antibody (MX Bv Ch Gt GP Ham Hs Ms Rb Rt Sh Serum Proteins) tested against purified Human IgG MX10. Each well was coated in duplicate with 1.0 µg of Human IgG (p/n 009-0102) [Red Line], Bovine IgG (p/n 001-0102) [Blue Line], Chicken IgG (003-0102), Goat IgG (p/n 005-0102) [Purple Line], Guinea Pig IgG (p/n 006-0102), Hamster IgG (p/n 007-0102), Horse IgG (p/n 008-0102), Mouse IgG (p/n 010-0102), Rabbit IgG (p/n 011-0102), Rat IgG (p/n 012-0102), Sheep IgG (p/n 013-0102). The working dilution is 1:184,500. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Donkey Anti-Goat IgG HRP conjugated (p/n 605-703-125) and TMB substrate (p/n TMBE-1000).