Goat IgG anti-Human IgG (Fc)-unconj., MinX none

Product Overview

• Article Name: Goat IgG anti-Human IgG (Fc)-unconj., MinX none
• Biozol Catalog Number: ROC-609-1103
• Supplier Catalog Number: 609-1103
• Alternative Catalog Number: ROC-609-1103
• Manufacturer: Rockland Immunochemicals
• Host: Goat
• Category: Antikörper
• Application: ELISA,IHC,WB
• Species Reactivity: Human
• Immunogen: Human IgG F(c) fragment
• Conjugation: Unconjugated
• Alternative Names: Goat Anti Human IgG Fc Antibody, Goat Anti-Human IgG F(c) Antibody, Goat Anti-Human IgG Fc Fragment Antibody
• Format: IgG
• Target Specificity: IgG (Fc)
• Cross-Adsorption (MinX): no cross-adsorbtion

Product Properties

• Clonality: Polyclonal
• Concentration: 5.17 mg/mL by UV absorbance at 280 nm
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Liquid (sterile filtered)
• Target: Human
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:20,000, IHC: 1:1,000 - 1:5,000, WB: 1:10,000 - 1:50,000
• Application Notes: Goat Anti-Human IgG F(c) Antibody has been tested by ELISA and dot blot and is suitable for western blot, immunohistochemistry, or immunoassays where specificity to the immunoglobulin Fc region is desired. Optimal concentrations in immunoassays should be

Images

ELISA Results of Goat Anti-Human IgG F(c) Antibody tested against purified Human IgG F(c). Each well was coated in duplicate with 1.0 µg of Human IgG F(c) (p/n 009-0103) [Red Line], Human IgG Fab (p/n 009-0105) [Green Line], Human IgG (p/n 009-0102) [Blue Line]. The working dilution is 1:143,000. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP Conjugation Stabilizer (p/n MB-076), Donkey Anti-Goat IgG HRP conjugated (p/n 605-703-125), and TMB substrate (p/n TMBE-1000).