Anti-Human IgA Alkaline Phosphatase Antibody has been tested by dot blot and ELISA and is assayed against 1.0 ug of Human IgA in a standard capture ELISA using pNPP p-nitrophenyl phosphate code NPP-10 as a substrate for 30 minutes at room temperature.
Dot Blot of Human IgA Antibody Alkaline Phosphatase Conjugated. Antigen: Human IgA. Load: Lane 1 - 100ng Lane 2 - 33.33ng Lane 3 - 11.11ng Lane 4 - 3.70ng Lane 5 - 1.23ng. Primary antibody: n/a Secondary antibody: Human IgA Antibody Alkaline Phosphatase Conjugated at 1:1,000 for 60 min at RT. Block: MB-070 for 60 min at RT. Visualized using Alkaline Phosphatase Substrate.
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