Goat IgG anti-Human IgG (F(ab)2)-Biotin, MinX none

Product Overview

• Article Name: Goat IgG anti-Human IgG (F(ab)2)-Biotin, MinX none
• Biozol Catalog Number: ROC-609-1604
• Supplier Catalog Number: 609-1604
• Alternative Catalog Number: ROC-609-1604
• Manufacturer: Rockland Immunochemicals
• Host: Goat
• Category: Antikörper
• Application: ELISA,IHC,WB
• Species Reactivity: Human
• Immunogen: Human IgG F(ab)2 fragment
• Conjugation: Biotin
• Alternative Names: Goat Anti Human IgG Fab2 Antibody Biotin Conjugated, Goat Anti-Human IgG F(ab)2 Antibody Biotin Conjugated, Goat Anti Human IgG Fab2 Fragment Antibody Biotin Conjugated
• Format: IgG
• Target Specificity: IgG (F(ab')2)
• Cross-Adsorption (MinX): no cross-adsorbtion

Product Properties

• Clonality: Polyclonal
• Concentration: 2.0 mg/mL by UV absorbance at 280 nm
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized
• Target: Human
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:20,000 - 1:500,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
• Application Notes: Anti-Human IgG F(ab)2 Biotin Antibody has been tested by ELISA and is ideal for ELISA, western blotting, Immunohistochemistry, Fluorescence Microscopy, Flow Cytometry as well as other antibody detection methods.

Images

ELISA of purified Goat Anti-Human IgG F(ab)2 Antibody Biotin Conjugated tested against purified Human IgG F(ab)2 Biotin. Each well was coated in duplicate with 1.0 µg of Human IgG F(ab)2 (p/n 009-0104). The working dilution is 1:108,000. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP Conjugate Stabilizer (p/n MB-076), Streptavidin-HRP conjugated (p/n S000-03) and TMB substrate (p/n TMBE-1000).