Goat IgG anti-Human IgG (H+L)-Biotin, MinX Ms, Polyclonal

Product Overview

• Article Name: Goat IgG anti-Human IgG (H+L)-Biotin, MinX Ms, Polyclonal
• Biozol Catalog Number: ROC-609-1616
• Supplier Catalog Number: 609-1616
• Alternative Catalog Number: ROC-609-1616
• Manufacturer: Rockland Immunochemicals
• Host: Goat
• Category: Antikörper
• Application: ELISA,IHC,WB
• Species Reactivity: Human
• Immunogen: Anti-Human IgG whole molecule was produced by repeated immunization with Human IgG whole molecule in goat.
• Conjugation: Biotin
• Alternative Names: Human Antibody, Gt-a-Human IgG, Human Antibody in Goat, Human Secondary Antibody
• Format: IgG
• Target Specificity: IgG (H+L)
• Cross-Adsorption (MinX): Mouse

Product Properties

• Clonality: Polyclonal
• Concentration: 2.0 mg/mL by UV absorbance at 280 nm
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized
• Target: Human
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:20,000 - 1:100,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
• Application Notes: Anti-Human IgG (H&L) Biotin Conjugated Antibody has been tested by ELISA and is suitable for immunoblotting (western or dot blot), ELISA, immunoelectron microscopy and immunohistochemistry as well as other antibody based enzymatic assays requiring lot-to

Images

ELISA Results of purified Goat Anti-Human IgG Antibody Biotin Conjugated (Min X Mouse Serum Proteins) tested against purified Human IgG Biotin. Each well was coated in duplicate with 1.0 µg of Human IgG (p/n 009-0102). The working dilution is 1:175,000. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP Conjugation Stabilizer (p/n MB-076), Donkey Anti-Goat IgG HRP conjugated (p/n 605-703-125) and TMB substrate (p/n TMBE-1000).