Mouse IgG anti-Human IgG (H+L)-HRPO, MinX none

Product Overview

• Article Name: Mouse IgG anti-Human IgG (H+L)-HRPO, MinX none
• Biozol Catalog Number: ROC-609-303-002
• Supplier Catalog Number: 609-303-002
• Alternative Catalog Number: ROC-609-303-002
• Manufacturer: Rockland Immunochemicals
• Host: Mouse
• Category: Antikörper
• Application: ELISA,IHC,WB
• Species Reactivity: Human
• Immunogen: Human IgG whole molecule
• Conjugation: HRPO
• Alternative Names: mouse anti-Human IgG peroxidase conjugated Antibody, mouse anti-Human IgG Antibody HRP conjugation
• Format: IgG
• Target Specificity: IgG (H+L)
• Cross-Adsorption (MinX): no cross-adsorbtion

Product Properties

• Clonality: Polyclonal
• Concentration: 1.0 mg/mL by UV absorbance at 280 nm
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized
• Target: Human
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:10,000 - 1:50,000, IHC: 1:500 - 1:2,500, WB: 1:1,000 - 1:5,000
• Application Notes: Anti-Human IgG Peroxidase conjugate has been tested by ELISA and is suitable for immunoblotting (western or dot blot), ELISA, immunoelectron microscopy and immunohistochemistry as well as other antibody-based enzymatic assays requiring lot-to-lot consist

Images

ELISA of purified Mouse Anti-Human IgG Antibody Peroxidase Conjugated tested against purified Human IgG HRP. Each well was coated in duplicate with 1.0 µg of Human IgG (p/n 009-0102). The working dilution is 1:28,000. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using TMB substrate (p/n TMBE-1000).