ATF2 phospho S490/phospho S498 Antibody, Rabbit, Polyclonal

Product Overview

• Article Name: ATF2 phospho S490/phospho S498 Antibody, Rabbit, Polyclonal
• Biozol Catalog Number: ROC-609-401-D11
• Supplier Catalog Number: 609-401-D11
• Alternative Catalog Number: ROC-609-401-D11
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Application: IHC, WB
• Species Reactivity: Human
• Immunogen: Anti-ATF2 pS490/pS498 Antibody was produced by repeated immunizations with synthetic phospho-peptide corresponding to amino acid residues surrounding Ser490/498.
• Conjugation: Unconjugated
• Alternative Names: rabbit anti-ATF2 pS490/pS498 Antibody, Cyclic AMP-dependent transcription factor ATF-2, Activating transcription factor 2, Cyclic AMP-responsive element-binding protein 2, CREB-2, HB16, cAMP response element-binding protein CRE-BP1

Product Properties

• Clonality: Polyclonal
• Concentration: Titrated value sufficient to run approximately 10 mini blots.
• NCBI: 1386
• UniProt: P15336
• Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5
• Form: Liquid
• Target: Human
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: ELISA: 1:10,000, IHC: 1:1000, WB: 1:1000
• Application Notes: Anti-ATF2 pS490/pS498 antibody is tested for use in ELISA, Western Blotting and IHC. Specific conditions for reactivity should be optimized by the end user. ATF2 antibody is specific for the approximately 74kDa ATF2 protein phosphorylated at Ser490,498.

Images

Western Blot of Rabbit Anti-ATF2 pS490/pS498 antibody. Lane 1: human melanoma cells with 0.0 µg/mL radiomimetic drug NCS. Lane 2: human melanoma cells with 0.1 µg/mL radiomimetic drug NCS. Lane 3: human melanoma cells with 0.3 µg/mL radiomimetic drug NCS. Lane 4: human melanoma cells with 0.5 µg/mL radiomimetic drug NCS. Lane 5: human melanoma cells with 1.0 µg/mL radiomimetic drug NCS. Primary antibody: ATF-2 antibody at 1:400 for overnight at 4C. Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: ~74kDa/~74kDa ATF2 protein phosphorylated at Ser490 and Ser498. Other band(s): none.