Parkin phospho S101 Antibody, Rabbit, Polyclonal

Product Overview

• Article Name: Parkin phospho S101 Antibody, Rabbit, Polyclonal
• Biozol Catalog Number: ROC-609-401-E06
• Supplier Catalog Number: 609-401-E06
• Alternative Catalog Number: ROC-609-401-E06
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Application: WB
• Species Reactivity: Human
• Immunogen: Anti-Parkin pS101 Antibody was produced by repeated immunizations with a synthetic phospho-peptide corresponding to amino acid residues surrounding Ser101.
• Conjugation: Unconjugated
• Alternative Names: rabbit anti-Parkin pS101 Antibody, E3 ubiquitin-protein ligase parkin, Parkin, Parkin RBR E3 ubiquitin-protein ligase, Parkinson juvenile disease protein 2, Parkinson disease protein 2, PRKN, PARK2

Product Properties

• Clonality: Polyclonal
• Concentration: Titrated value sufficient to run approximately 10 mini blots.
• NCBI: 5071
• UniProt: O60260
• Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5
• Form: Liquid
• Target: Human
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: WB: 1:1000
• Application Notes: Anti-Parkin pS101 Antibody is tested for use in Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 52 kDa in size corresponding to parking protein phosphorylated at Ser101 in the appro

Images

Western Blot of Rabbit anti-Parkin pS101 antibody. Lane 1: HEK293 cells transfected with Parkin WT (Phospho). Lane 2: HEK293 cells transfected with Parkin S101 mutant (non-phospho). Load: 10 µg per lane. Primary antibody: Parkin pS101 antibody at 1:1,000 for overnight at 4C. Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 52 kDa for Parkin pS101. Other band(s): none.