Rabbit IgG anti-Human IgG (F(ab)2)-unconj., MinX Ms, Polyclonal , Unconjugated

Product Overview

• Article Name: Rabbit IgG anti-Human IgG (F(ab)2)-unconj., MinX Ms, Polyclonal , Unconjugated
• Biozol Catalog Number: ROC-609-4118
• Supplier Catalog Number: 609-4118
• Alternative Catalog Number: ROC-609-4118
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Application: ELISA,IHC,WB
• Species Reactivity: Human
• Immunogen: Anti-Human IgG F(ab)2 fragment was produced in rabbit by repeated immunization with human IgG F(ab)2 fragment.
• Conjugation: Unconjugated
• Alternative Names: F(ab)2 Human IgG Antibody, Rb-a-Human F(ab)2 IgG, F(ab)2 Human IgG Antibody in Rabbit, Pre-Adsorbed Human IgG Secondary Antibody
• Format: IgG
• Target Specificity: IgG (F(ab')2)
• Cross-Adsorption (MinX): Mouse

Product Properties

• Clonality: Polyclonal
• Concentration: 2.0mg/mL by UV absorbance at 280 nm
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Liquid (sterile filtered)
• Target: Human
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:350,000, IHC: 1:1,000 - 1:5,000, WB: 1:3,000 - 1:15,000
• Application Notes: Anti-Human IgG F(ab)2 antibody has been tested by ELISA and dot blot and is suitable for western blotting, IHC and for ELISA. Researchers should determine optimal titers for applications that are not stated below.

Images

ELISA Results of Rabbit Anti-Human IgG F(ab)2 Antibody (Min X Mouse Serum Proteins) tested against purified Human IgG F(ab)2 Mx Ms. Each well was coated in duplicate with 1.0 µg of Human IgG F(ab)2 (p/n 009-0104) [Red Line], Human IgG (p/n 009-0102) [Green Line], and Mouse IgG (p/n 010-0102) [Blue Line]. The working dilution of Human IgG F(ab)2 MX is 1:56300. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP Conjugation Stabilizer (p/n MB-076), Goat Anti-Rabbit HRP conjugated (p/n 611-103-122), and TMB substrate (p/n TMBE-1000).