Rabbit IgG anti-Human IgG (H+L)-HRPO, MinX none

Product Overview

• Article Name: Rabbit IgG anti-Human IgG (H+L)-HRPO, MinX none
• Biozol Catalog Number: ROC-609-4302
• Supplier Catalog Number: 609-4302
• Alternative Catalog Number: ROC-609-4302
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Application: ELISA,IHC,WB
• Species Reactivity: Human
• Immunogen: Anti-Human IgG whole molecule was produced by repeated immunization with Human IgG whole molecule in rabbit.
• Conjugation: HRPO
• Alternative Names: rabbit anti-Human IgG Antibody peroxidase Conjugation, rabbit anti-Human IgG HRP Conjugated Antibody
• Format: IgG
• Target Specificity: IgG (H+L)
• Cross-Adsorption (MinX): no cross-adsorbtion

Product Properties

• Clonality: Polyclonal
• Concentration: 2.0 mg/mL by UV absorbance at 280 nm
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized
• Target: Human
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:150,000 - 1:250,000, IHC: 1:1,000 - 1:5,000, WB: 1:5,000 - 1:25,000
• Application Notes: Anti-Human IgG (H&L) Peroxidase Conjugated Antibody has been tested by ELISA and Dot Blot and is suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody

Images

Dot Blot Results of Rabbit Anti-Human IgG HRP Conjugated Antibody. Human IgG sample loaded 1- 100ng, 2- 33.3ng, 3- 11.1ng, 4- 3.7ng, 5- 1.2ng. Rabbit Anti-Human IgG Peroxidase Conjugated Antibody 1:1000 for 60mins at RT. Blocking Buffer for Fluorescent Western Blotting (p/n MB-070) for 1hr at RT.