Rabbit IgG anti-Human IgG (Fc)-HRPO, MinX none

Product Overview

• Article Name: Rabbit IgG anti-Human IgG (Fc)-HRPO, MinX none
• Biozol Catalog Number: ROC-609-4303
• Supplier Catalog Number: 609-4303
• Alternative Catalog Number: ROC-609-4303
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Application: ELISA,IHC,WB
• Species Reactivity: Human
• Immunogen: Human IgG F(c) fragment
• Conjugation: HRPO
• Alternative Names: rabbit Anti-Human IgG F(c) Antibody peroxidase Conjugated, rabbit Anti-Human IgG Fc fragment Antibody peroxidase Conjugated, rabbit Anti-Human IgG Fc Antibody HRP Conjugated
• Format: IgG
• Target Specificity: IgG (Fc)
• Cross-Adsorption (MinX): no cross-adsorbtion

Product Properties

• Clonality: Polyclonal
• Concentration: 2.0 mg/mL by UV absorbance at 280 nm
• Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized
• Target: Human
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:10,000 - 1:50,000, IHC: 1:500 - 1:2,500, WB: 1:1,000 - 1:5,000
• Application Notes: Anti-Human IgG F(c) Peroxidase Conjugated Antibody has been tested by ELISA and is suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymati

Images

ELISA results of purified Rabbit anti-Human IgG F(c) Antibody Peroxidase Conjugated tested against purified Human IgG F(c). Each well was coated in duplicate with 1.0 µg of Human IgG F(c) (p/n 009-0103). The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gelatin as blocking buffer and TMB substrate p/n TMBE-1000.