Rabbit IgG anti-Human IgG (H+L)-HRPO, MinX Ms, Polyclonal

Product Overview

• Article Name: Rabbit IgG anti-Human IgG (H+L)-HRPO, MinX Ms, Polyclonal
• Biozol Catalog Number: ROC-609-4317
• Supplier Catalog Number: 609-4317
• Alternative Catalog Number: ROC-609-4317
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Application: ELISA,IHC,WB
• Species Reactivity: Human
• Immunogen: Anti-Human IgG (H&L) was produced by repeated immunization with human IgG whole molecule in rabbit.
• Conjugation: HRPO
• Alternative Names: rabbit anti-Human IgG Antibody peroxidase Conjugation, rabbit anti-Human IgG HRP Conjugated Antibody
• Format: IgG
• Target Specificity: IgG (H+L)
• Cross-Adsorption (MinX): Mouse

Product Properties

• Clonality: Polyclonal
• Concentration: 2.0 mg/mL by UV absorbance at 280 nm
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized
• Target: Human
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:50,000 - 1:100,000, IHC: 1:500 - 1:2,500, WB: 1:1,000 - 1:5,000
• Application Notes: Anti-Human IgG HRP has been tested by ELISA and is suitable for immunoblotting, western blot, dot blot, ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot

Images

ELISA results of purified Rabbit Anti-Human IgG Peroxidase Conjugated Antibody (Min X mouse serum) tested against purified Human IgG. Each well was coated in duplicate with 1.0 µg of Human IgG (red line). The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using TMB-1000 substrate.