Rabbit IgG anti-Human IgG (H+L)-Alk. Phos., MinX none, ALP, Polyclonal , AP

Product Overview

• Article Name: Rabbit IgG anti-Human IgG (H+L)-Alk. Phos., MinX none, ALP, Polyclonal , AP
• Biozol Catalog Number: ROC-609-4502
• Supplier Catalog Number: 609-4502
• Alternative Catalog Number: ROC-609-4502
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Application: ELISA,IHC,WB
• Species Reactivity: Human
• Immunogen: Human IgG whole molecule
• Conjugation: Alk. Phos.
• Alternative Names: rabbit anti-Human IgG Antibody Alkaline Phosphatase Conjugation, rabbit-anti-Human IgG Alkaline Phosphatase Conjugated Antibody
• Format: IgG
• Target Specificity: IgG (H+L)
• Cross-Adsorption (MinX): no cross-adsorbtion

Product Properties

• Clonality: Polyclonal
• Concentration: 1.0 mg/mL by UV absorbance at 280 nm
• Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol, pH 8.0
• Form: Liquid (sterile filtered)
• Target: Human
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:2,000 - 1:10,000, IHC: 1:200 - 1:1,000, WB: 1:500 - 1:2,500
• Application Notes: Anti-Human IgG Alkaline Phosphatase Antibody has been tested by ELISA and dot blot and is suitable for ELISA, Western Blot and immunohistochemistry applications. Specific conditions for reactivity should be optimized by the end user.

Images

Dot Blot of Rabbit anti-Human IgG Antibody Alkaline Phosphatase Conjugated. Antigen: Human IgG. Load: Lane 1 - 200 ng Lane 2 - 66.7 ng Lane 3 - 22.2 ng Lane 4 - 7.41 ng Lane 5 - 2.47 ng. Primary antibody: n/a. Secondary antibody: Rabbit anti-Human IgG Antibody Alkaline Phosphatase Conjugated at 1:1,000 for 1 HR at RT. Block: MB-070 for 1 HR at RT.