Rabbit IgG anti-Human IgG (H)-Alk. Phos., MinX none, ALP, Polyclonal , AP

Product Overview

• Article Name: Rabbit IgG anti-Human IgG (H)-Alk. Phos., MinX none, ALP, Polyclonal , AP
• Biozol Catalog Number: ROC-609-4512
• Supplier Catalog Number: 609-4512
• Alternative Catalog Number: ROC-609-4512
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Application: ELISA,IHC,WB
• Species Reactivity: Human
• Immunogen: Human IgG gamma heavy chain
• Conjugation: Alk. Phos.
• Alternative Names: rabbit anti-Human IgG (gamma chain) alkaline phosphatase conjugated Antibody, rabbit anti-Human IgG Antibody alk phos conjugation
• Format: IgG
• Target Specificity: IgG (Fc)
• Cross-Adsorption (MinX): no cross-adsorbtion

Product Properties

• Clonality: Polyclonal
• Concentration: 1.0 mg/mL by UV absorbance at 280 nm
• Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol, pH 8.0
• Form: Liquid (sterile filtered)
• Target: Human
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:10,000 - 1:20,000, IHC: 1:200 - 1:1,000, WB: 1:500 - 1:2,500
• Application Notes: Anti-Human IgG (gamma chain) Alkaline Phosphatase has been tested by dot blot and ELISA and is assayed against 1.0 ug of Human IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate code NPP-10 as a substrate for 30 minutes at room temperat

Images

Dot Blot of Rabbit Anti-Human IgG gamma chain Alkaline Phosphatase Conjugated Antibody. Lane 1: 100ng hu IgG. Lane 2: 33.3ng hu IgG. Lane 3: 11.1ng hu IgG. Lane 4: 3.70ng hu IgG. Lane 5: 1.23ng hu IgG. Secondary Antibody: Rt-a-Hu IgG Alk Phos at 1µg/mL. Blocking Buffer: BlockOut MB-073 for 30 min at RT.