Rabbit IgG anti-Human IgG (H)-Biotin, MinX none

Product Overview

• Article Name: Rabbit IgG anti-Human IgG (H)-Biotin, MinX none
• Biozol Catalog Number: ROC-609-4612
• Supplier Catalog Number: 609-4612
• Alternative Catalog Number: ROC-609-4612
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Application: ELISA,IHC,WB
• Species Reactivity: Human
• Immunogen: Human IgG gamma heavy chain
• Conjugation: Biotin
• Alternative Names: Rabbit Anti Human IgG (gamma chain) Biotin Conjugated Antibody, Rabbit Anti-Human IgG Antibody Biotin Conjugation, Rabbit Anti-Human IgG (gamma chain) Antibody Biotin Conjugate
• Format: IgG
• Target Specificity: IgG (Fc)
• Cross-Adsorption (MinX): no cross-adsorbtion

Product Properties

• Clonality: Polyclonal
• Concentration: 1.0 mg/mL by UV absorbance at 280 nm
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized
• Target: Human
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:20,000 - 1:100,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
• Application Notes: Anti-Human IgG (gamma chain) Biotin Conjugation has been tested by ELISA and is assayed against 1.0 ug of Human IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin S000-03 and ABTS (2,2-azino-bis-[3-ethylbenthiazoline-6-sulfonic ac

Images

ELISA Results of Rabbit Anti-Human IgG Antibody Biotin Conjugated tested against purified Human IgG Biotin. Each well was coated in duplicate with 1.0 µg of Human IgG (p/n 009-0102). The working dilution is 1:34600. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP Conjugate Stabilizer (p/n MB-076), Streptavidin-HRP conjugated (p/n S000-03), and TMB substrate (p/n TMBE-1000).