Rabbit IgG anti-Human IgG (H+L)-Biotin, MinX Ms, Polyclonal

Product Overview

• Article Name: Rabbit IgG anti-Human IgG (H+L)-Biotin, MinX Ms, Polyclonal
• Biozol Catalog Number: ROC-609-4617
• Supplier Catalog Number: 609-4617
• Alternative Catalog Number: ROC-609-4617
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Application: ELISA,IHC,WB
• Species Reactivity: Human
• Immunogen: Human IgG whole molecule
• Conjugation: Biotin
• Alternative Names: Rabbit Anti Human IgG Biotin Conjugated Antibody, Rabbit Anti-Human IgG Antibody Biotin Conjugation
• Format: IgG
• Target Specificity: IgG (H+L)
• Cross-Adsorption (MinX): Mouse

Product Properties

• Clonality: Polyclonal
• Concentration: 2.0 mg/mL by UV absorbance at 280 nm
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized
• Target: Human
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:20,000 - 1:100,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
• Application Notes: Anti-Human IgG (H&L) Biotin has been tested by ELISA and western blot and has been assayed against 1.0 ug of Human IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin S000-03 and ABTS (2,2-azino-bis-[3-ethylbenthiazoline-6-sulfonic

Images

Preservation efficacy of surface-bound (A-C) S1 protein and (D-F) N protein stored for different durations with ZIF-90 and without ZIF-90 encapsulation. (A-F) n=8, ****P < 0.0001, analyzed by unpaired t-test. Biotin-labeled anti human IgG was used as secondary antibody. Figure 4. PMID: 34297470.

Western Blot of Biotin Conjugated Rabbit Anti-Human IgG Pre-Adsorbed Secondary Antibody. Lane 1: Human IgG. Lane 2: None. Load: 50 ng per lane. Primary antibody: Human IgG Antibody biotin conjugated at 1:1,000 for 60 min at RT. Secondary antibody: Peroxidase streptavidin secondary antibody at 1:40,000 for 30 min at RT. Blocking: MB-070 for 30 min at RT. Predicted/Observed size: 25 & 55 kDa, 25 & 55 kDa for Human IgG. Other band(s): None.