Rabbit Anti Human IgG Fab2 Antibody Biotin Conjugated, Rabbit Anti-Human IgG F(ab)2 Antibody Biotin Conjugated, Rabbit Anti Human IgG Fab2 Fragment Antibody Biotin Conjugated
Format:
IgG
Target Specificity:
IgG (F(ab')2)
Cross-Adsorption (MinX):
Mouse
Clonality:
Polyclonal
Concentration:
1.0 mg/mL by UV absorbance at 280 nm
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Anti-Human IgG F(ab)2 Biotin Antibody has been tested by ELISA and is assayed against 1.0 ug of Human IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin S000-03 and ABTS (2,2-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code
ELISA Results of Rabbit Anti-Human IgG F(ab)2 Antibody Biotin Conjugated (Min X Mouse Serum Proteins) tested against purified Human IgG F(ab)2 Biotin. Each well was coated in duplicate with 1.0 µg of Human IgG F(ab)2 (p/n 009-0104). The working dilution is 1:75000. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP Conjugation Stabilizer (p/n MB-076), Streptavidin-HRP conjugated (p/n S000-03), and TMB substrate (p/n TMBE-1000).
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