Donkey IgG anti-Human IgG (H+L)-HRPO, MinX none

Product Overview

• Article Name: Donkey IgG anti-Human IgG (H+L)-HRPO, MinX none
• Biozol Catalog Number: ROC-609-703-002
• Supplier Catalog Number: 609-703-002
• Alternative Catalog Number: ROC-609-703-002
• Manufacturer: Rockland Immunochemicals
• Host: Donkey
• Category: Antikörper
• Application: ELISA,IHC,WB
• Species Reactivity: Human
• Immunogen: Human IgG whole molecule
• Conjugation: HRPO
• Alternative Names: donkey anti-Human IgG peroxidase conjugated Antibody, donkey anti-Human IgG Antibody HRP conjugation
• Format: IgG
• Target Specificity: IgG (H+L)
• Cross-Adsorption (MinX): no cross-adsorbtion

Product Properties

• Clonality: Polyclonal
• Concentration: 2.0 mg/mL by UV absorbance at 280 nm
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized
• Target: Human
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:10,000 - 1:50,000, IHC: 1:500 - 1:2,500, WB: 1:1,000 - 1:10,000
• Application Notes: Anti-Human IgG (H&L) Peroxidase Conjugated Antibody has been tested by ELISA and is assayed against 1.0 µg of Human IgG in a standard capture ELISA using ABTS (2,2-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code ABTS-100 as a substrate for 30 m

Images

ELISA Results of Purified Donkey Anti-Human IgG Peroxidase Conjugated Antibody tested against purified Human IgG protein. Each well was coated in duplicate with 10 µg of Human IgG (p/n 009-0102). The working dilution of Anti-Human IgG HRP is 1:30,000. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% Fish Gel/PBS Blocking buffer (p/n MB-066) and TMBE-1000 substrate.