Donkey IgG anti-Human IgG (H+L)-Alk. Phos., MinX Bo,Ck,Go,Gp,Hm,Ho,Ms,Rb,Rt,Sh, ALP, Polyclonal , AP

Product Overview

• Article Name: Donkey IgG anti-Human IgG (H+L)-Alk. Phos., MinX Bo,Ck,Go,Gp,Hm,Ho,Ms,Rb,Rt,Sh, ALP, Polyclonal , AP
• Biozol Catalog Number: ROC-609-705-123
• Supplier Catalog Number: 609-705-123
• Alternative Catalog Number: ROC-609-705-123
• Manufacturer: Rockland Immunochemicals
• Host: Donkey
• Category: Antikörper
• Application: ELISA,IHC,WB
• Species Reactivity: Human
• Immunogen: Human IgG whole molecule
• Conjugation: Alk. Phos.
• Alternative Names: donkey anti-Human IgG alkaline phosphatase conjugated Antibody, donkey anti-Human IgG Antibody alk phos conjugation
• Format: IgG
• Target Specificity: IgG (H+L)
• Cross-Adsorption (MinX): Bovine,Gallus,Goat,Guinea pig,Hamster (all),Equine,Mouse,Rabbit,Rat,Sheep

Product Properties

• Clonality: Polyclonal
• Concentration: 1.0 mg/mL by UV absorbance at 280 nm
• Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol, pH 8.0
• Form: Liquid (sterile filtered)
• Target: Human
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:2,000 - 1:10,000, IHC: 1:200 - 1:1,000, WB: 1:500 - 1:2,500
• Application Notes: Anti-Human IgG Alkaline Phosphatase conjugate has been tested by ELISA and is suitable for immunoblotting (western or dot blot), ELISA, immunoelectron microscopy and immunohistochemistry as well as other antibody-based enzymatic assays requiring lot-to-l

Images

ELISA results of Purified Donkey Anti-Human IgG MinX10 Antibody Alkaline Phosphatase Conjugated tested against purified Human IgG mx10. Each well was coated in duplicate with 10µg of Human IgG (p/n 009-0102). The working dilution of Anti-Human IgG mx10 Alkaline Phosphatase Antibody is 1:34,000. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% Fish Gel Blocking buffer (p/n MB-066) and NPP Substrate (p/n NPP-100).