Chicken IgG anti-Human IgG (H+L)-unconj., MinX none, Gallus, Polyclonal , Unconjugated

Product Overview

• Article Name: Chicken IgG anti-Human IgG (H+L)-unconj., MinX none, Gallus, Polyclonal , Unconjugated
• Biozol Catalog Number: ROC-609-9102
• Supplier Catalog Number: 609-9102
• Alternative Catalog Number: ROC-609-9102
• Manufacturer: Rockland Immunochemicals
• Host: Gallus
• Category: Antikörper
• Application: ELISA,IHC,WB
• Species Reactivity: Human
• Immunogen: Human IgG whole molecule
• Conjugation: Unconjugated
• Alternative Names: Chicken Anti Human IgG Antibody, Chicken Anti-Human IgG Antibody
• Format: IgG
• Target Specificity: IgG (H+L)
• Cross-Adsorption (MinX): no cross-adsorbtion

Product Properties

• Clonality: Polyclonal
• Concentration: 2.04 mg/mL by UV absorbance at 280 nm
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Liquid (sterile filtered)
• Target: Human
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:20,000 - 1:60,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
• Application Notes: Anti-Human IgG antibody has been tested by ELISA and is suitable for western blot and immunohistochemistry, as well as other assays requiring lot-to-lot consistency.

Images

ELISA Results of Purified Chicken Anti-Human IgG Antibody tested against purified Human IgG. Each well was coated in duplicate with 10 µg of Human IgG (p/n 009-0102). The working dilution of Anti-Human IgG is 1:110,000. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% Fish Gel/PBS Blocking buffer (p/n MB-066), Goat Anti-Chicken IgG mx10 HRP conjugated (p/n 603-103-126) and TMB substrate (p/n TMBE-1000).