Chicken IgG anti-Human IgG (H+L)-Alk. Phos., MinX none, ALP, Gallus, Polyclonal , AP

Product Overview

• Article Name: Chicken IgG anti-Human IgG (H+L)-Alk. Phos., MinX none, ALP, Gallus, Polyclonal , AP
• Biozol Catalog Number: ROC-609-9502
• Supplier Catalog Number: 609-9502
• Alternative Catalog Number: ROC-609-9502
• Manufacturer: Rockland Immunochemicals
• Host: Gallus
• Category: Antikörper
• Application: ELISA,IHC,WB
• Species Reactivity: Human
• Immunogen: Human IgG whole molecule
• Conjugation: Alk. Phos.
• Alternative Names: chicken anti-Human IgG alkaline phosphatase conjugated Antibody, chicken anti-Human IgG Antibody alk phos conjugation
• Format: IgG
• Target Specificity: IgG (H+L)
• Cross-Adsorption (MinX): no cross-adsorbtion

Product Properties

• Clonality: Polyclonal
• Concentration: 1.0 mg/mL by UV absorbance at 280 nm
• Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol, pH 8.0
• Form: Liquid (sterile filtered)
• Target: Human
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:2,000 - 1:10,000, IHC: 1:200 - 1:1,000, WB: 1:500 - 1:2,500
• Application Notes: Anti-Human IgG (H&L) Alkaline Phosphatase has been tested by western blot and assayed against 1.0 ug of Human IgG in a standard capture ELISA using pNPP (p-nitrophenyl phosphate code NPP-10) as a substrate for 30 minutes at room temperature. A working

Images

Western Blot of Chicken anti-Human IgG Antibody Alkaline Phosphatase Conjugated Lane 1: Human IgG Load: 100 ng per lane Secondary antibody: Human IgG (H&L) Antibody Alkaline Phosphatase Conjugated at 1:1,000 for 60 min at RT Block: MB-070 for 30 min at RT Predicted/Observed size: 55 and 28 kDa, 55 kDa