Chicken IgG anti-Human IgG (H+L)-Biotin, MinX none, Gallus, Polyclonal

Product Overview

• Article Name: Chicken IgG anti-Human IgG (H+L)-Biotin, MinX none, Gallus, Polyclonal
• Biozol Catalog Number: ROC-609-9602
• Supplier Catalog Number: 609-9602
• Alternative Catalog Number: ROC-609-9602
• Manufacturer: Rockland Immunochemicals
• Host: Gallus
• Category: Antikörper
• Application: ELISA,IHC,WB
• Species Reactivity: Human
• Immunogen: Human IgG whole molecule
• Conjugation: Biotin
• Alternative Names: Chicken Anti Human IgG Antibody Biotin Conjugate, Chicken Anti-Human IgG Biotin conjugated Antibody
• Format: IgG
• Target Specificity: IgG (H+L)
• Cross-Adsorption (MinX): no cross-adsorbtion

Product Properties

• Clonality: Polyclonal
• Concentration: 1.0 mg/mL by UV absorbance at 280 nm
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized
• Target: Human
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:20,000 - 1:100,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
• Application Notes: This product has been assayed against 1.0 ug of Human IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin S000-03 and ABTS (2,2-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code ABTS-100 as a substrate for 30 minutes at room

Images

IgG responses to SARS-CoV-2 N protein variants. Four SARS-CoV-2 N protein variants were fabricated on to the microarray surface, including full-length N protein, core domain (amino acids 44-364), C-terminal domain (CTD) (amino acids 248-365) and N terminal domain (NTD) (amino acids 24-181). The IgG response from the plasma of 8 colorectal cancer (C) and 7 convalescent COVID-19 (P) patients were assessed for the 4 variants. Figure S5. PMID: 33925055.

Linearity of signal as a function of serum dilution on the microarray platform. Panels A & B show data for the N core domains, N-terminal domain and C-terminal domain, for patients 189 and 192 respectively. Panels C & D show data for peptides 6, 8 & 16, for patients 189 and 192 respectively. x-axis shows the serum dilutions for each measurement. y-axis units are relative fluorescence units (RFU). Figure S4. PMID: 33925055.