Goat IgG anti-Mouse IgG2b (Fc)-unconj., MinX none

Product Overview

• Article Name: Goat IgG anti-Mouse IgG2b (Fc)-unconj., MinX none
• Biozol Catalog Number: ROC-610-101-042
• Supplier Catalog Number: 610-101-042
• Alternative Catalog Number: ROC-610-101-042
• Manufacturer: Rockland Immunochemicals
• Host: Goat
• Category: Antikörper
• Application: ELISA,IHC,WB
• Species Reactivity: Mouse
• Immunogen: Mouse IgG2b heavy chain
• Conjugation: Unconjugated
• Alternative Names: goat anti-MOUSE IgG2b Antibody, goat anti-Mouse IgG2b (gamma 2b) Antibody
• Format: IgG
• Target Specificity: IgG2b (Fc)
• Cross-Adsorption (MinX): no cross-adsorbtion

Product Properties

• Clonality: Polyclonal
• Concentration: 1.0 mg/mL by UV absorbance at 280 nm
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Liquid (sterile filtered)
• Target: Mouse
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:40,000 - 1:200,000, IHC: 1:2,000 - 1:10,000, WB: 1:4,000 - 1:20,000
• Application Notes: Mouse IgG2b secondary antibody has been tested by dot blot and ELISA and is available in a variety of formats. Anti-Mouse IgG2b secondary antibody is suitable for ELISA, Immunohistochemistry, western blotting as well as other anti IgG2b antibody based as

Images

ELISA Results of Goat Anti-Mouse IgG2b (Gamma 2b chain) Antibody Minimum Cross Reactivity to Bovine, Human, and Rabbit Serum Proteins tested against purified Mouse IgG2b mx3. Each well was coated in duplicate with 1.0 µg of Mouse IgG2b (p/n 010-0142). The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP Conjugate Stabilizer (p/n MB-076), Donkey Anti-Goat IgG HRP conjugated (p/n 605-703-125), and TMB substrate (p/n TMBE-1000).