Goat IgG anti-Mouse IgG (H+L)-unconj., MinX Bo,Ck,Go,Gp,Hm,Ho,Hu,Rb,Rt,Sh, Polyclonal , Unconjugated

Product Overview

• Article Name: Goat IgG anti-Mouse IgG (H+L)-unconj., MinX Bo,Ck,Go,Gp,Hm,Ho,Hu,Rb,Rt,Sh, Polyclonal , Unconjugated
• Biozol Catalog Number: ROC-610-101-121
• Supplier Catalog Number: 610-101-121
• Alternative Catalog Number: ROC-610-101-121
• Manufacturer: Rockland Immunochemicals
• Host: Goat
• Category: Antikörper
• Application: ELISA,IHC,WB
• Species Reactivity: Mouse
• Immunogen: Anti-Mouse IgG whole molecule was produced by repeated immunization with Mouse IgG whole molecule in goat.
• Conjugation: Unconjugated
• Alternative Names: goat anti-Mouse IgG Antibody, Gt-a-Mouse IgG, Mouse Antibody in Goat, Mouse Secondary Antibody
• Format: IgG
• Target Specificity: IgG (H+L)
• Cross-Adsorption (MinX): Bovine,Gallus,Goat,Guinea pig,Hamster (all),Equine,Human,Rabbit,Rat,Sheep

Product Properties

• Clonality: Polyclonal
• Concentration: 0.95 mg/mL by UV absorbance at 280 nm
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Liquid (sterile filtered)
• Target: Mouse
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:50,000 - 1:100,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:20,000
• Application Notes: Anti-Mouse IgG has been tested by ELISA and is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal detection should be optimized by the end u

Images

ELISA Results of Goat Anti-Mouse IgG (H&L) Antibody (Min X Bv Ch Gt GP Ham Hs Hu Rb Rt & Sh Serum Proteins) tested against purified Mouse IgG mx10. Each well was coated in duplicate with 1.0 µg of Mouse IgG (p/n 010-0102) [Red Line], Bovine IgG (p/n 001-0102) [Green Line], Chicken IgG (p/n 003-0102) [Blue Line], Goat IgG (p/n 005-0102) [Purple Line], Guinea Pig IgG (p/n 006-0102), Hamster IgG (p/n 007-0102), Horse IgG (p/n 008-0102), Human IgG (p/n 009-0102), Rabbit IgG (p/n 011-0102), Rat IgG (p/n 012-0102), and Sheep IgG (p/n 013-0102). The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using TMB substrate (p/n TMBE-1000).