Goat IgG anti-Mouse IgG+IgM (H+L)-HRPO, MinX none

Product Overview

• Article Name: Goat IgG anti-Mouse IgG+IgM (H+L)-HRPO, MinX none
• Biozol Catalog Number: ROC-610-103-115
• Supplier Catalog Number: 610-103-115
• Alternative Catalog Number: ROC-610-103-115
• Manufacturer: Rockland Immunochemicals
• Host: Goat
• Category: Antikörper
• Application: ELISA,IHC,WB
• Species Reactivity: Mouse
• Immunogen: Anti-Mouse IgG and IgM whole molecule was produced by repeated immunization with Mouse IgG and IgM whole molecule in goat.
• Conjugation: HRPO
• Alternative Names: Mouse IgG and IgM whole molecule Antibody, Gt-a-Mouse IgG and IgM whole molecule Peroxidase conjugated, Mouse IgG and IgM whole molecule Peroxidase Antibody in goat, Mouse IgG and IgM whole molecules peroxidase conjugated Secondary Antibody.
• Format: IgG
• Target Specificity: IgG+IgM (H+L)
• Cross-Adsorption (MinX): no cross-adsorbtion

Product Properties

• Clonality: Polyclonal
• Concentration: 1.0 mg/mL by UV absorbance at 280 nm
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized
• Target: Mouse
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:40,000, IHC: 1:500 - 1:2,500, WB: 1:2,000 - 1:10,000
• Application Notes: Anti-Mouse IgG and IgM Peroxidase conjugated antibody has been tested by ELISA and is suitable for highly specific immunological methods requiring extremely low background levels, lot-to-lot consistency, high titer and specificity.

Images

ELISA Results of Goat Anti-Mouse IgG + IgM Antibody Peroxidase Conjugated Minimum Cross Reactivity to Bovine, Horse and Human Serum Proteins tested against purified Mouse IgG + IgM. Each well was coated in duplicate with 10 µg of Mouse IgG (p/n 010-0102) [Red Line], Mouse IgM (IgG/IgA free) [Green Line], and Mouse IgG/IgM (p/n 010-0102/010-0107) [Blue Line]. The working dilution is 1:175,000. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% Fish Gel/PBS Blocking buffer (p/n MB-066), and TMB substrate (p/n TMBE-1000).