Goat IgG anti-Mouse IgG (H+L)-unconj., MinX Hu, Polyclonal , Unconjugated

Product Overview

• Article Name: Goat IgG anti-Mouse IgG (H+L)-unconj., MinX Hu, Polyclonal , Unconjugated
• Biozol Catalog Number: ROC-610-1119
• Supplier Catalog Number: 610-1119
• Alternative Catalog Number: ROC-610-1119
• Manufacturer: Rockland Immunochemicals
• Host: Goat
• Category: Antikörper
• Application: ELISA,IHC,WB
• Species Reactivity: Mouse
• Immunogen: Mouse IgG whole molecule
• Conjugation: Unconjugated
• Alternative Names: Goat Anti Mouse IgG (H&L) Antibody Pre-Adsorbed, Goat Anti-Mouse IgG Antibody Pre-Adsorbed
• Format: IgG
• Target Specificity: IgG (H+L)
• Cross-Adsorption (MinX): Human

Product Properties

• Clonality: Polyclonal
• Concentration: 8.6 mg/mL by UV absorbance at 280 nm
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Liquid (sterile filtered)
• Target: Mouse
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:100,000 - 1:500,000, IHC: 1:5,000 - 1:25,000, WB: 1:10,000 - 1:50,000
• Application Notes: Anti-Mouse IgG antibody has been tested by ELISA and is suitable for ELISA, western blot, and immunohistochemistry, as well as other assays requiring lot-to-lot consistency.

Images

ELISA results of purified Goat anti-Mouse IgG Antibody Min X human serum tested against purified Mouse IgG. Each well was coated in duplicate with 1.0 µg of Mouse IgG [red line], Human IgG [green line]. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Blocking buffer MB-060-1000, Gt-anti-Donkey HRP conjugate 616-1302, and TMB-1000 substrate.