Goat IgG anti-Mouse IgG (H+L)-HRPO, MinX Hu, Polyclonal

Product Overview

• Article Name: Goat IgG anti-Mouse IgG (H+L)-HRPO, MinX Hu, Polyclonal
• Biozol Catalog Number: ROC-610-1319-0500
• Supplier Catalog Number: 610-1319-0500
• Alternative Catalog Number: ROC-610-1319-0500
• Manufacturer: Rockland Immunochemicals
• Host: Goat
• Category: Antikörper
• Application: ELISA,IHC,WB
• Species Reactivity: Mouse
• Immunogen: Mouse IgG whole molecule
• Conjugation: HRPO
• Alternative Names: Goat Anti-Mouse IgG Secondary Antibody Peroxidase Conjugated, Goat Anti-Mouse IgG Secondary Antibody HRP Conjugated, GAM-HRP, Anti-mouse secondary antibody, anti-mouse HRP antibody, horseradish peroxidase conjugated secondary antibody, anti-mouse horsera
• Format: IgG
• Target Specificity: IgG (H+L)
• Cross-Adsorption (MinX): Human

Product Properties

• Clonality: Polyclonal
• Concentration: 1.0 mg/mL by UV absorbance at 280 nm
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized
• Target: Mouse
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:100,000, IHC: User Optimized, WB: 1:2,000 - 1:10,000
• Application Notes: Mouse secondary antibody conjugated to horseradish peroxidase is available in a variety of formats. Anti-Mouse IgG Peroxidase Antibody has been tested by ELISA and is suitable for ELISA, Immunohistochemistry, western blotting, as well as other peroxidase

Images

ELISA results of purified Goat anti-Mouse IgG Antibody Peroxidase Conjugated (Min x Human Serum Proteins) tested against purified Mouse IgG. Each well was coated in duplicate with 1.0 µg of Mouse IgG (p/n 009-0102). The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gelatin and TME ELISA Peroxidase Substrate (p/n TMBE-1000).