Goat IgG anti-Mouse IgG3 (Fc)-ATTO 594, MinX none, ATTO-594, Polyclonal

Product Overview

• Article Name: Goat IgG anti-Mouse IgG3 (Fc)-ATTO 594, MinX none, ATTO-594, Polyclonal
• Biozol Catalog Number: ROC-610-155-043
• Supplier Catalog Number: 610-155-043
• Alternative Catalog Number: ROC-610-155-043
• Manufacturer: Rockland Immunochemicals
• Host: Goat
• Category: Antikörper
• Application: FLISA,FACS,IF,WB
• Species Reactivity: Mouse
• Immunogen: Mouse IgG3 heavy chain
• Conjugation: ATTO 594
• Alternative Names: goat anti-MOUSE IgG3 Antibody ATTO594 Conjugation, goat anti-MOUSE IgG3 ATTO 594 Conjugated Antibody
• Format: IgG
• Target Specificity: IgG3 (Fc)
• Cross-Adsorption (MinX): no cross-adsorbtion

Product Properties

• Clonality: Polyclonal
• Concentration: 1.0 mg/mL by UV absorbance at 280 nm
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized
• Target: Mouse
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: FLISA: >1:20,000, Flow Cytometry: 1:500 - 1:2,500, IF Microscopy: >1:5,000, WB: 1:4,000 - 1:20,000
• Application Notes: Anti-Mouse IgG3 ATTO 594 Antibody has been tested by dot blot and is available in a variety of formats. ATTO dye conjugates are designed for STED microscopy, FRET, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent wes

Images

ATTO dyes can be used for multicolor immunofluorescent detection with low background and high signal. Examples shown are: A. Tubulin in PtK2- male Rat Kangaroo Kidney Epithelial Cells was detected using ATTO 532 labeled secondary antibody. B. Muscle alpha-actin was stained with a mouse primary antibody and ATTO 488 anti-mouse IgG (green) while Cytokeratin was stained with polyclonal rabbit anti-cytokeratin and ATTO 647N anti-rabbit IgG (red). C. HUVEC (Human umbilical vein endothelial cells were stained with anti- Vimentin-ATTO 532 (green), anti-E-Cadherin-ATTO 655 (red) and DAPI (blue). D. Rat colon sections were stained with Anti-Aquaporin 3-ATTO 594 antibody. Hoechst 33342 (blue) is used as counterstain. Images provided courtesy of Dr. Jörg Reichwein, ATTO-TEC GmbH