Goat IgG anti-Mouse IgG (H+L)-Biotin, MinX none

Product Overview

• Article Name: Goat IgG anti-Mouse IgG (H+L)-Biotin, MinX none
• Biozol Catalog Number: ROC-610-1602
• Supplier Catalog Number: 610-1602
• Alternative Catalog Number: ROC-610-1602
• Manufacturer: Rockland Immunochemicals
• Host: Goat
• Category: Antikörper
• Application: ELISA,IHC,WB
• Species Reactivity: Mouse
• Immunogen: Anti-Mouse IgG was produced by repeated immunization with Mouse IgG whole molecule in goat.
• Conjugation: Biotin
• Alternative Names: Goat Anti-Mouse IgG Secondary Antibody biotin Conjugation, Goat Anti-Mouse IgG Antibody biotin Conjugated, Anti-mouse IgG secondary antibody, anti-mouse IgG biotin conjugated secondary antibody
• Format: IgG
• Target Specificity: IgG (H+L)
• Cross-Adsorption (MinX): no cross-adsorbtion

Product Properties

• Clonality: Polyclonal
• Concentration: 2.0 mg/mL by UV absorbance at 280 nm
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized
• Target: Mouse
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:350,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:20,000
• Application Notes: Anti-Mouse IgG (H&L) biotin conjugated antibody has been tested by ELISA and is suitable for highly specific immunological methods requiring extremely low background levels, lot-to-lot consistency, high titer and specificity.

Images

ELISA results of purified Goat anti-Mouse IgG Antibody Biotin Conjugated tested against purified Mouse IgG. Each well was coated in duplicate with 1.0 µg of Mouse IgG (p/n 010-0102). The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gelatin as blocking buffer, Streptavidin Peroxidase Conjugated p/n S000-03) and TMB substrate p/n TMBE-1000.