Anti-Mouse IgG subclass pan reactive Texas Red Antibody has been tested by western blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. Anti-mouse IgG antibody Texas Red (TM) is al
Immunofluorescence of Rabbit Anti-Mouse IgG (gamma 1, 2a, 2b and 3 chain) Antibody Texas Red(TM) Conjugated. Cell line: HeLa. Primary Antibody: Alpha Tubulin (p/n 200-301-880) at 4 µg/mL (1:250) for 1hr at RT. Secondary Antibody: Rabbit Anti-Mouse IgG exas Red(TM) (p/n 610-409-C49) at 1 µg/mL (1:1000) overnight at 4 C. Fixative: Ice Cold Methanol. Permeabilization: Ice Cold Methanol. Nuclear stain: Hoechst 33342. Expected Localization: Cytoplasmic. Image: A) Alpha Tubulin, B) Nuclear Stain, C) Merge, D) Secondary Only Control.
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