0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form:
Lyophilized
Target:
Mouse
Antibody Type:
Secondary Antibody
Application Dilute:
FLISA: 1:10,000 - 1:50,000, Flow Cytometry: User Optimized, IF Microscopy: 1:1,000 - 1:5,000
Application Notes:
Anti-Mouse IgG Fluorescein Antibody has been tested by dot blot and western blot and is designed for flow cytometry, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable fo
Western blot of Fluorescein conjugated Rabbit Anti-Mouse IgG secondary antibody. Lane 1: Mouse IgG. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Fluorescein rabbit secondary antibody at 1:1,000 for 60 min at RT. Blocking: MB-070 for 30 min at RT. Predicted/Observed size: 25 & 55 kDa, 25 & 55 kDa for Mouse IgG. Other band(s): None.
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