Rabbit IgG anti-Mouse IgM (µ)-HRPO, MinX none

Product Overview

• Article Name: Rabbit IgG anti-Mouse IgM (µ)-HRPO, MinX none
• Biozol Catalog Number: ROC-610-4307
• Supplier Catalog Number: 610-4307
• Alternative Catalog Number: ROC-610-4307
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Application: ELISA,IHC,WB
• Species Reactivity: Mouse
• Immunogen: Mouse IgM whole molecule
• Conjugation: HRPO
• Alternative Names: Rabbit Anti-Mouse IgM (mu chain) Antibody peroxidase Conjugated, Rabbit Anti-Mouse IgM mu chain Antibody HRP Conjugation
• Format: IgG
• Target Specificity: IgM (µ)
• Cross-Adsorption (MinX): no cross-adsorbtion

Product Properties

• Clonality: Polyclonal
• Concentration: 1.5 mg/mL by UV absorbance at 280 nm
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized
• Target: Mouse
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:180,000, IHC: 1:500 - 1:2,500, WB: 1:1,000 - 1:5,000
• Application Notes: Mouse IgM (mu chain) peroxidase conjugated antibody has been tested by ELISA and is suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymat

Images

ELISA Results of Rabbit Anti-Mouse IgM Antibody Peroxidase Conjugated tested against purified Mouse IgM HRP. Each well was coated in duplicate with 1.0 µg of Mouse IgM (p/n 010-0107). The working dilution is 1:190,000. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using TMB substrate (p/n TMBE-1000).