Rabbit IgG anti-Mouse IgG1 (Fc)-HRPO, MinX none

Product Overview

• Article Name: Rabbit IgG anti-Mouse IgG1 (Fc)-HRPO, MinX none
• Biozol Catalog Number: ROC-610-4340
• Supplier Catalog Number: 610-4340
• Alternative Catalog Number: ROC-610-4340
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Application: ELISA,IHC,WB
• Species Reactivity: Mouse
• Immunogen: Mouse IgG1 heavy chain
• Conjugation: HRPO
• Alternative Names: Rabbit Anti-Mouse IgG1 (Gamma 1 chain) Antibody peroxidase Conjugation, Rabbit Anti-Mouse IgG1 HRP Conjugated Antibody
• Format: IgG
• Target Specificity: IgG1 (Fc)
• Cross-Adsorption (MinX): no cross-adsorbtion

Product Properties

• Clonality: Polyclonal
• Concentration: 1.0 mg/mL by UV absorbance at 280 nm
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized
• Target: Mouse
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:20,000 - 1:200,000, IHC: 1:500 - 1:2,500, WB: 1:2,000 - 1:20,000
• Application Notes: Mouse IgG1 secondary antibody conjugated to HRP is available in a variety of formats. Anti-Mouse IgG1 Peroxidase Antibody has been tested by ELISA and western blot and is suitable for ELISA, Immunohistochemistry western blotting as well as other anti IgG

Images

Western blot of Peroxidase conjugated Rabbit Anti-Mouse IgG1 (Gamma 1 chain) secondary antibody. Lane 1: Mouse IgG1. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Peroxidase rabbit secondary antibody at 1:1,000 for 60 min at RT. Blocking: MB-070 for 30 min at RT. Predicted/Observed size: 55 kDa, 55 kDa for Mouse IgG1 (Gamma 1 chain). Other band(s): None.