Rabbit IgG anti-Mouse IgM (µ)-Alk. Phos., MinX none, ALP, Polyclonal , AP

Product Overview

• Article Name: Rabbit IgG anti-Mouse IgM (µ)-Alk. Phos., MinX none, ALP, Polyclonal , AP
• Biozol Catalog Number: ROC-610-4507
• Supplier Catalog Number: 610-4507
• Alternative Catalog Number: ROC-610-4507
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Application: ELISA,IHC,WB
• Species Reactivity: Mouse
• Immunogen: Mouse IgM whole molecule
• Conjugation: Alk. Phos.
• Alternative Names: Rabbit Anti-Mouse IgM (mu chain) Antibody alkaline phosphatase conjugated, Rabbit Anti-Mouse IgM mu chain Antibody alk phos Conjugation
• Format: IgG
• Target Specificity: IgM (µ)
• Cross-Adsorption (MinX): no cross-adsorbtion

Product Properties

• Clonality: Polyclonal
• Concentration: 1.0 mg/mL by UV absorbance at 280 nm
• Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol, pH 8.0
• Form: Liquid (sterile filtered)
• Target: Mouse
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:20,000, IHC: User Optimized, WB: User Optimized
• Application Notes: Anti-MOUSE IgM (alpha chain) Alkaline Phosphatase Antibody has been tested by ELISA and is suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based

Images

ELISA Results of Rabbit Anti-Mouse IgM Antibody Alkaline Phosphatase Conjugate tested against purified Mouse IgM Alk Phos. Each well was coated in duplicate with 1.0 µg of Mouse IgM (p/n 010-0107). The working dilution is 1:6700. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using ELISA Alkaline Phosphatase Substrate Buffer (p/n NPP-10) and NPP Working Buffer (p/n NPP-B500).