Rabbit IgG anti-Mouse IgG (H+L)-Alk. Phos., MinX Hu, ALP, Polyclonal , AP

Product Overview

• Article Name: Rabbit IgG anti-Mouse IgG (H+L)-Alk. Phos., MinX Hu, ALP, Polyclonal , AP
• Biozol Catalog Number: ROC-610-4520
• Supplier Catalog Number: 610-4520
• Alternative Catalog Number: ROC-610-4520
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Application: ELISA,IHC,WB
• Species Reactivity: Mouse
• Immunogen: Mouse IgG whole molecule
• Conjugation: Alk. Phos.
• Alternative Names: rabbit anti-Mouse IgG Antibody alkaline phosphatase conjugation, rabbit anti-Mouse IgG alk phos conjugated Antibody
• Format: IgG
• Target Specificity: IgG (H+L)
• Cross-Adsorption (MinX): Human

Product Properties

• Clonality: Polyclonal
• Concentration: 1.0 mg/mL by UV absorbance at 280 nm
• Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol, pH 8.0
• Form: Liquid (sterile filtered)
• Target: Mouse
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:9,800, IHC: 1:100 - 1:500, WB: 1:500 - 1:2,000
• Application Notes: Anti-Mouse IgG Alkaline Phosphatase conjugate has been tested by ELISA and western blot and is suitable for immunoblotting (western or dot blot), ELISA, immunoelectron microscopy and immunohistochemistry as well as other antibody-based enzymatic assays r

Images

Western Blot of Alkaline Phosphatase Conjugated Rabbit anti-Mouse IgG antibody. Lane 1: Mouse IgG. Lane 2: none. Load: 100 ng per lane. Primary antibody: none. Secondary antibody: Alkaline Phosphatase mouse secondary antibody at 1:1,000 for 60 min at RT. Block: MB-070 for 30 min RT. Predicted/Observed size: 55 kDa, 28 kDa for Mouse IgG. Other band(s): none.