Rabbit IgG anti-Mouse IgG (F(ab)2)-Biotin, MinX none

Product Overview

• Article Name: Rabbit IgG anti-Mouse IgG (F(ab)2)-Biotin, MinX none
• Biozol Catalog Number: ROC-610-4604
• Supplier Catalog Number: 610-4604
• Alternative Catalog Number: ROC-610-4604
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Application: ELISA,IHC,WB
• Species Reactivity: Mouse
• Immunogen: Mouse IgG F(ab)2 fragment
• Conjugation: Biotin
• Alternative Names: Rabbit Anti-Mouse IgG F(ab)2 Antibody Biotin Conjugate, Rabbit Anti Mouse IgG Fab2 Biotin Conjugated Antibody, Rabbit Anti Mouse IgG Fab2 Fragment Antibody Biotin Conjugation
• Format: IgG
• Target Specificity: IgG (F(ab')2)
• Cross-Adsorption (MinX): no cross-adsorbtion

Product Properties

• Clonality: Polyclonal
• Concentration: 2.0 mg/mL by UV absorbance at 280 nm
• Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized
• Target: Mouse
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:20,000 - 1:100,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
• Application Notes: Anti-Mouse IgG F(ab)2 Biotin antibody has been tested by ELISA and dot blot and is assayed against 1.0 µg of Mouse IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin S000-03 and ABTS (2,2-azino-bis-[3-ethylbenthiazoline-6-sulfonic

Images

ELISA results of purified Rabbit Anti-Mouse IgG F(ab)2 Biotin Conjugated Antibody tested against purified Mouse IgG F(ab)2. Each well was coated in duplicate with 1.0 µg of Mouse IgG F(ab)2 (blue line). The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Blocking buffer MB-060-1000, Streptavidin HRP conjugate, and TMB substrate.