Rabbit IgG anti-Mouse IgG1 (Fc)-Biotin, MinX none

Product Overview

• Article Name: Rabbit IgG anti-Mouse IgG1 (Fc)-Biotin, MinX none
• Biozol Catalog Number: ROC-610-4640
• Supplier Catalog Number: 610-4640
• Alternative Catalog Number: ROC-610-4640
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Application: ELISA,IHC,WB
• Species Reactivity: Mouse
• Immunogen: Mouse IgG1 heavy chain
• Conjugation: Biotin
• Alternative Names: Rabbit Anti-Mouse IgG1 (Gamma 1 chain) Antibody biotin Conjugation, Rabbit Anti-Mouse IgG1 BAC Conjugated Antibody
• Format: IgG
• Target Specificity: IgG1 (Fc)
• Cross-Adsorption (MinX): no cross-adsorbtion

Product Properties

• Clonality: Polyclonal
• Concentration: 1.0 mg/mL by UV absorbance at 280 nm
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized
• Target: Mouse
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:250,000, IHC: 1:500 - 1:3,000, WB: 1:1,000 - 1:5,000
• Application Notes: Mouse IgG1 secondary antibody conjugated to Biotin is available in a variety of formats. Anti-Mouse IgG1 Biotin Antibody has been tested by ELISA and western blot and is suitable for ELISA, Immunohistochemistry western blotting as well as other anti IgG1

Images

ELISA Results of Rabbit Anti-Mouse IgG1 Antibody Biotin Conjugation tested against purified Mouse IgG1 Biotin. Each well was coated in duplicate with 1.0 µg of Mouse IgG1 (p/n 010-0140). The working dilution is 1:146,000. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP Conjugate Stabilizer (p/n MB-076), Streptavidin-HRP conjugated (p/n S000-03) and TMB substrate (p/n TMBE-1000).