This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platfor
Isolated hADSCs (IADSCs) were differentiated into SMCs using retinoic acid (RA), heparin was used as a positive control, while SK-UT-1 cells was used as a SMC control. Expression of SMC markers smooth muscle alpha actin (SM-alphaa, red, Texas Red Conjugated anti-Mouse IgG2, gamma2a chain specific, p/n 610-4941), desmin (green, Fluorescein Conjugated anti-Mouse IgG1, gamma1 chain specific, p/n 610-4240), smooth muscle myosin heavy chain (SM-MHC, red, Texas Red Conjugated anti-Mouse kappa, kappa chain specific, p/n 610-4910), and smoothelin (green, Fluorescein Conjugated anti-Mouse IgG1, gamma1 chain specific, p/n 610-4240) in differentiated SMCs was determined by indirect immunofluorescence. Nuclei were counter stained with DAPI (blue). Expression of all four markers can be seen in all the cells, particularly in RA differentiated SMCs. Fig. 5. PMID: 21373882.
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